abstract：:By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts by pores designed to...

journal_title：Nature biotechnology

authors： Russo MJ,Bayley H,Toner M

更新日期：1997-03-01 00:00:00

abstract：:CD4 functions as a major T-cell surface receptor for human immunodeficiency virus by binding the human immunodeficiency virus type 1 (HIV-1) envelope protein gp120 with relatively high affinity. We have developed constrained aromatically modified analogs of the secondary structures of the first domain of CD4 in order ...

journal_title：Nature biotechnology

authors： Zhang X,Gaubin M,Briant L,Srikantan V,Murali R,Saragovi U,Weiner D,Devaux C,Autiero M,Piatier-Tonneau D,Greene MI

更新日期：1997-02-01 00:00:00

abstract：:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were s...

journal_title：Nature biotechnology

authors： Kuwabara I,Maruyama H,Mikawa YG,Zuberi RI,Liu FT,Maruyama IN

更新日期：1997-01-01 00:00:00

abstract：:The human genome encodes approximately 100,000 different genes, and at least partial sequence information for nearly all will be available soon. Sequence information alone, however, is insufficient for a full understanding of gene function, expression, regulation, and splice-site variation. Because cellular processes ...

journal_title：Nature biotechnology

authors： Lockhart DJ,Dong H,Byrne MC,Follettie MT,Gallo MV,Chee MS,Mittmann M,Wang C,Kobayashi M,Horton H,Brown EL

更新日期：1996-12-01 00:00:00

abstract：:Mycobacterium tuberculosis is a natural mutant in oxyR, a close homolog of the central regulator of peroxide stress response in enteric bacteria. Inactivation of oxyR is specific for M. tuberculosis and other members of the M. tuberculosis complex. This phenomenon appears as a paradox due to the ability of this organi...

journal_title：Nature biotechnology

authors： Deretic V,Pagán-Ramos E,Zhang Y,Dhandayuthapani S,Via LE

更新日期：1996-11-01 00:00:00

abstract：:Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...

journal_title：Nature biotechnology

authors： Palukaitis P,Roossinck MJ

更新日期：1996-10-01 00:00:00

abstract：:The potential therapeutic applications of encapsulated cells are enormous. In the US alone, it has been estimated that nearly half-a-trillion dollars are spent each year to care for patients who suffer tissue loss or dysfunction. Over 6 million patients suffer from neurodegenerative disorders such as Alzheimer's disea...

journal_title：Nature biotechnology

authors： Lanza RP,Hayes JL,Chick WL

更新日期：1996-09-01 00:00:00

abstract：:The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cu...

journal_title：Nature biotechnology

authors： Altpeter F,Vasil V,Srivastava V,Vasil IK

更新日期：1996-09-01 00:00:00

abstract：:A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...

journal_title：Nature biotechnology

authors： Ishizaki-Nishizawa O,Fujii T,Azuma M,Sekiguchi K,Murata N,Ohtani T,Toguri T

更新日期：1996-08-01 00:00:00

abstract：:We describe a novel approach to design a set of primers selective for large groups of genes. This method is based on the distribution frequency of all nucleotide combinations (octa- to decanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect genes. By analyzing 1000 human ...

journal_title：Nature biotechnology

authors： López-Nieto CE,Nigam SK

更新日期：1996-07-01 00:00:00

abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...

journal_title：Nature biotechnology

authors： Schöpke C,Taylor N,Cárcamo R,Konan NK,Marmey P,Henshaw GG,Beachy RN,Fauquet C

更新日期：1996-06-01 00:00:00

abstract：:Tyrosine phosphorylation exerts a pivotal role in cell regulation processes of higher eukaryotes. Tight control of the activity of protein tyrosine kinases is crucial for ordered phosphorylation to occur. We have developed a functional screen for tyrosine kinase regulators using c-Src, the first cellular protein tyros...

journal_title：Nature biotechnology

authors： Superti-Furga G,Jönsson K,Courtneidge SA

更新日期：1996-05-01 00:00:00

abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...

journal_title：Nature biotechnology

authors： Moore JC,Arnold FH

更新日期：1996-04-01 00:00:00

abstract：:We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization d...

journal_title：Nature biotechnology

authors： Tyagi S,Kramer FR

更新日期：1996-03-01 00:00:00